Preliminary characterization of a geminivirus in tomatillo (physalis ixocarpa b.) in the central region of Mexico

Document Type

Article

Publication Date

12-1-2002

Abstract

A geminivirus infecting tomatillo (Physalis ixocarpa B.) plants collected in Uriels in the States of Mexico, Puebla and Morelos was detected and biologically characterized. The geminiviruses present in the samples were analyzed by agarose gel electrophoresis, molecular hybridization (using PHV coat protein gene as probe), and a PCR-based strategy that involving the amplification and sequencing of a genomic fragment that included part of the intergenic region. The analysis indicated that the geminiviruses isolated from several samples from tomatillo plants were similar to PHV. The presence of other, uncharacterized geminiviruses could not be discarded. The percentage of geminivirus-infected plants was rather low compared with the percentage of plants infected with RNA viruses, such as Cucumber mosaic (CMV), Tobacco mosaic (TMV), Tobacco etch (TEV), Tobacco ringspot (TRSV) and Tomato spotted wilt (TSWV). In addition, the analysis of ds-RNA patterns indicated the presence of several unknown RNA viruses in some tomatillo plants.

Publication Source (Journal or Book title)

Agrocencia

First Page

471

Last Page

481

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