Comparative inhibitory activities of sulforaphane and phenethyl isothiocyanate against leukemia resistant CEM/C2 cancer cells
Document Type
Article
Publication Date
4-1-2009
Abstract
The potentials of sulforaphane and phenethyl isothiocyanate as functional food ingredients against hematological malignancies were evaluated on the intracellular targets of these antiangiogenic compounds in CEM/C2 T leukemia cells. CEM/C2 cells were seeded and incubated with various concentrations of sulforaphane or phenethyl isothiocyanate for 24 or 48 h. Sulforaphane at 0-30 μmol/L dose-dependently suppressed CEM/C2 cell growth and proliferation and caused cell death by apoptosis with down-regulation of bcl-2 and increased release of cytochrome c within 48 h. Phenethyl isothiocyanate at 0-15 μmol/L dose-dependently inhibited CEM/C2 cell viability within 48 h and caused cell death by apoptosis with down-regulation of bcl-2 and increased expression of cytochrome c. CEM/C2 cell death was accompanied by inhibition of IκB phosphorylation and degradation and nuclear translocation of p65-nuclear factor kappaB (NF-κB) by sulforaphane as well as phenethyl isothiocyanate. CEM/C2 cell viability and proliferation can be inhibited by functional food ingredient inhibitors of the NF-κB pathway. © 2009 Elsevier Ltd. All rights reserved.
Publication Source (Journal or Book title)
Journal of Functional Foods
First Page
229
Last Page
235
Recommended Citation
Losso, J., & Truax, R. (2009). Comparative inhibitory activities of sulforaphane and phenethyl isothiocyanate against leukemia resistant CEM/C2 cancer cells. Journal of Functional Foods, 1 (2), 229-235. https://doi.org/10.1016/j.jff.2009.02.003