Title
Flow cytometric and immunohistochemical detection of in vivo BrdU-labeled cells in mouse fat depots
Document Type
Article
Publication Date
1-16-2009
Abstract
This study has determined the natural frequency and localization of progenitor/stem cells within fat depots in situ based on their ability to retain DNA nucleotide label (BrdU). Neonate and mature male C57BL6/J mice were injected intraperitoneally with BrdU- and label-retaining cells (LRC) were quantified in fat depots by immunohistochemical, immunofluorescent, and flow cytometric methods. In neonates, LRC constituted 27% of the cells in inguinal fat (iWAT) and 65% in interscapular brown fat (BAT) after Day 10 and 26% of the cells in epididymal fat (eWAT) after Day 28. After 52 days, the LRC accounted for 0.72% of iWAT, 0.53% of eWAT and 1.05% of BAT, respectively. The BrdU-labeled cells localized to two areas: single cells distributed among adipocytes or those adjacent to the blood vessels wall. In mature C57BL6/J mice, flow cytometric analysis determined that a majority of the LRC were also positive for stem cell antigen-1 (Sca-1). © 2008 Elsevier Inc. All rights reserved.
Publication Source (Journal or Book title)
Biochemical and Biophysical Research Communications
First Page
539
Last Page
544
Recommended Citation
Staszkiewicz, J., Gimble, J., Cain, C., Dietrich, M., Burk, D., Kirk-Ballard, H., & Gawronska-Kozak, B. (2009). Flow cytometric and immunohistochemical detection of in vivo BrdU-labeled cells in mouse fat depots. Biochemical and Biophysical Research Communications, 378 (3), 539-544. https://doi.org/10.1016/j.bbrc.2008.11.092
