Title
An alternative pathway for Alu retrotransposition suggests a role in DNA double-strand break repair
Document Type
Article
Publication Date
3-1-2009
Abstract
The Alu family is a highly successful group of non-LTR retrotransposons ubiquitously found in primate genomes. Similar to the L1 retrotransposon family, Alu elements integrate primarily through an endonuclease-dependent mechanism termed target site-primed reverse transcription (TPRT). Recent studies have suggested that, in addition to TPRT, L1 elements occasionally utilize an alternative endonuclease-independent pathway for genomic integration. To determine whether an analogous mechanism exists for Alu elements, we have analyzed three publicly available primate genomes (human, chimpanzee and rhesus macaque) for endonuclease-independent recently integrated or lineage specific Alu insertions. We recovered twenty-three examples of such insertions and show that these insertions are recognizably different from classical TPRT-mediated Alu element integration. We suggest a role for this process in DNA double-strand break repair and present evidence to suggest its association with intra-chromosomal translocations, in-vitro RNA recombination (IVRR), and synthesis-dependent strand annealing (SDSA). © 2008 Elsevier Inc. All rights reserved.
Publication Source (Journal or Book title)
Genomics
First Page
205
Last Page
212
Recommended Citation
Srikanta, D., Sen, S., Huang, C., Conlin, E., Rhodes, R., & Batzer, M. (2009). An alternative pathway for Alu retrotransposition suggests a role in DNA double-strand break repair. Genomics, 93 (3), 205-212. https://doi.org/10.1016/j.ygeno.2008.09.016
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