The systemic immune response induced by nasal immunization of inactivated SARS virus in BALB/c mice

Document Type

Article

Publication Date

5-1-2004

Abstract

Objective: To understand the immune mechanism of the β-propiolactone inactivated SARS virus in BALB/c mice by nasal immunization. Methods: Five-week-old female BALB/c mice were immunized with a single dose of 50μg of the inactivated virus through nasal immunization. The control mice were given equal volume of PBS. Five mice of each group were killed on day 8 and 14, and lymphocytes from the spleen and peripheral blood were isolated. The lymphocyte phenotypes were detected by FACS. The specific antibody IgM and IgG were detected by ELISA. Results: The splenocyte CD4+ T cells significantly increased on day 8 (P = 0.027). The peripheral blood lymphocyte phenotype began to change on day 8, but on day 14 the phenotype changed significantly in the immunized mice. The CD4+, CD8+, CD3+ T cells and the ratio of the T cells to B cells significantly decreased than those in the control mice (P < 0.05, 0.01, 0.01 and 0.01, respectively); whereas, the CD45R/B220+ cells (B cells) significantly increased (P = 0.000). The serum IgM and IgG antibody were not detected. Conclusion: The inactivated SARS virus without any adjuvant immunized by nasal route could make the lymphocyte phenotype change in BALB/c mice, but couldn't induce the mice to generate antibody.

Publication Source (Journal or Book title)

Chinese Journal of Microbiology and Immunology

First Page

409

Last Page

411

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