Purification of nanoparticle PCR products and their topography observed with AFM

Authors

Lijuan Mi, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Bin Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Hualan Zhou, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Huabin Wang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Jun Hu, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Document Type

Article

Publication Date

10-1-2007

Abstract

Nanoparticle PCR (NP-PCR) is a new method to optimize PCR amplification. Suitable amount of Au nanoparticles can improve specificity, sensitivity and extension rate of PCR. In this paper, we compare efficiency of purifying NP-PCR products with different methods. In addition, topographies of DNA products in NP-PCR were observed with atomic force microscope (AFM). The results show that most of DNA products purified directly by routing method remain almost free due to less effect of nanoparticales. The yields decrease when the AuNPs were removed by high-speed centrifugation. A little amount of DNA subsided with AuNPs.

Publication Source (Journal or Book title)

He Jishu/Nuclear Techniques

First Page

876

Last Page

880

Recommended Citation

Mi, L., Li, B., Zhou, H., Wang, H., & Hu, J. (2007). Purification of nanoparticle PCR products and their topography observed with AFM. He Jishu/Nuclear Techniques, 30 (10), 876-880. Retrieved from https://repository.lsu.edu/ag_exst_pubs/774