Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG

Authors

Peng Wang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Junhong Lu, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Xinyan Wang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Haijun Yang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Huabin Wang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Yibo Huang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Bin Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Document Type

Article

Publication Date

7-1-2008

Abstract

Atomic force microscope (AFM) is a powerful tool for virus studies. The recombinant adeno-associated virus serotype 2 (rAAV2) has an intendancy to aggregate in liquid or on solid surface. Moreover, changes in virus shape induced by compressing force of the AFM tip hamper studies on virus topography. In this paper, chemical reagent of glutaraldehyde (2%, v/v) pre-mixed with virus suspension facilitates the dispersion of rAAV2 particles deposited on both the mica and HOPG surface. In addition, the height of virus particle is up to near 20 nm with the increase of concentration of glutaraldehyde.

Publication Source (Journal or Book title)

He Jishu/Nuclear Techniques

First Page

557

Last Page

560

Recommended Citation

Wang, P., Lu, J., Wang, X., Yang, H., Wang, H., Huang, Y., & Li, B. (2008). Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG. He Jishu/Nuclear Techniques, 31 (7), 557-560. Retrieved from https://repository.lsu.edu/ag_exst_pubs/718