Functionalized inorganic-organic composite material derivated by sol-gel for construction of mediated amperometric hydrogen peroxide biosensor
Document Type
Article
Publication Date
5-3-1999
Abstract
A novel functionalized inorganic-organic hybrid material with cation exchange property was prepared by sol-gel method. The H2O2 biosensor was fabricated by simply dipping the horseradish peroxidase-containing functionalized membrane modified electrode into Meldola's blue (MDB) solution. MDB was adsorbed and firmly immobilized within the membrane. The electrochemical behavior of MDB incorporated in the membrane was more reversible compared with that of the solution species and suitable as mediator for the horseradish peroxidase. The response time was less than 25s. Linear range is up to 0.6mM (Corr. coeff. 0.9998) with detection limit of 9x10-7M. High sensitivity of 75nAμMcm-2 was obtained due to high MDB-loading. The biosensor exhibited a good stability. Copyright (C) 1999 Elsevier Science B.V.
Publication Source (Journal or Book title)
Analytica Chimica Acta
First Page
71
Last Page
78
Recommended Citation
Zhang, J., Li, B., Wang, Z., Cheng, G., & Dong, S. (1999). Functionalized inorganic-organic composite material derivated by sol-gel for construction of mediated amperometric hydrogen peroxide biosensor. Analytica Chimica Acta, 388 (1-2), 71-78. https://doi.org/10.1016/S0003-2670(99)00043-4