Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium

Authors

Bingquan Wang, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences
Bin Li, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences
Zhenxin Wang, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences
Quobao Xu, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences
Qun Wang, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences
Shaojun Dong, Changchun Institute Of Applied Chemistry, Chinese Academy Of Sciences

Document Type

Article

Publication Date

5-15-1999

Abstract

An acid-stable soybean-peroxidase biosensor was developed by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 μA/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).

Publication Source (Journal or Book title)

Analytical Chemistry

First Page

1935

Last Page

1939

Recommended Citation

Wang, B., Li, B., Wang, Z., Xu, Q., Wang, Q., & Dong, S. (1999). Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium. Analytical Chemistry, 71 (10), 1935-1939. https://doi.org/10.1021/ac980940q