Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells

Authors

Pedro P. Carvalho, Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808, USA.
Xiying Wu
Gang Yu
Marilyn Dietrich
Isabel R. Dias
Manuela E. Gomes
Rui L. Reis
Jeffrey M. Gimble

Document Type

Article

Publication Date

5-1-2011

Abstract

Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.

Publication Source (Journal or Book title)

Cytotherapy

First Page

594

Last Page

7

Recommended Citation

Carvalho, P. P., Wu, X., Yu, G., Dietrich, M., Dias, I. R., Gomes, M. E., Reis, R. L., & Gimble, J. M. (2011). Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. Cytotherapy, 13 (5), 594-7. https://doi.org/10.3109/14653249.2010.544721