Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis

Authors

Gail Kilroy, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Marilyn Dietrich, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Xiying Wu, LaCell, Inc., New Orleans, LA, USA.
Jeffrey M. Gimble, LaCell, Inc., New Orleans, LA, USA.
Z Elizabeth Floyd, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA. Elizabeth.Floyd@pbrc.edu.

Document Type

Article

Publication Date

1-1-2018

Abstract

Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells along with protocols for inducing adipogenesis in this cell population or isolating the adipose stromal vascular fraction cells for flow cytometric analysis.

Publication Source (Journal or Book title)

Methods in molecular biology (Clifton, N.J.)

First Page

137

Last Page

146

Recommended Citation

Kilroy, G., Dietrich, M., Wu, X., Gimble, J. M., & Floyd, Z. E. (2018). Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis. Methods in molecular biology (Clifton, N.J.), 1773, 137-146. https://doi.org/10.1007/978-1-4939-7799-4_11