miR-196a expression in human and canine osteosarcomas: a comparative study

Laura Pazzaglia, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy. Electronic address: laura.pazzaglia@ior.it.
Leonardo Leonardi, Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Amalia Conti, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
Chiara Novello, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
Irene Quattrini, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
Luisa Montanini, Department of Paediatrics, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
Franco Roperto, Department of Biology, University "Federico II" of Napoli, Via Delpino 1, 80126 Napoli, Italy.
Fabio Del Piero, Department of Pathobiological Sciences, School of Veterinary Medicine - Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.
Giovanni Di Guardo, Faculty of Veterinary Medicine, University of Teramo, Viale Crucioli, 122, 64100 Teramo, Italy.
Federica Piro, Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Piero Picci, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
Maria Serena Benassi, Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.

Document Type

Article

Publication Date

4-1-2015

Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumour in dogs and humans. MicroRNAs are short non-coding RNA molecules involved in post-transcriptional gene expression. Here, we compared the effects of miR-196a deregulation in human and canine OS cells after having observed a more uniform distribution and stronger down-expression in the human specimens. Cell response to miR-196a transfection was different in human and canine OS. A decreased proliferation rate was seen in human MG63 and 143B OS cells, while no appreciable changes occurred in canine DAN cells. Transient decrease of motility was highly remarkable and longer in MG63, concomitant with decreased levels of annexin1, a target of miR-196a promoting cell migration and invasion. In conclusion, the effects of miR-196a over-expression on tumour cell response may be strictly related to species and cell type. Further studies are needed to define the impact of miRNA deregulation on OS development.

Publication Source (Journal or Book title)

Research in veterinary science

First Page

112

Last Page

9

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