Cyanidin Stimulates Insulin Secretion and Pancreatic β-Cell Gene Expression through Activation of l-type Voltage-Dependent Ca Channels

Authors

Tanyawan Suantawee, Program in Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand. suantawee@gmail.com.
Sara T. Elazab, Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. sara.taha@ymail.com.
Walter H. Hsu, Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. whsu@iastate.edu.
Shaomian Yao, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. shaomia@lsu.edu.
Henrique Cheng, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. hcheng@lsu.edu.
Sirichai Adisakwattana, Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Sirichai.a@chula.ac.th.

Document Type

Article

Publication Date

7-28-2017

Abstract

Cyanidin is a natural anthocyanidin present in fruits and vegetables with anti-diabetic properties including stimulation of insulin secretion. However, its mechanism of action remains unknown. In this study, we elucidated the mechanisms of cyanidin for stimulatory insulin secretion from pancreatic β-cells. Rat pancreatic β-cells INS-1 were used to investigate the effects of cyanidin on insulin secretion, intracellular Ca signaling, and gene expression. We detected the presence of cyanidin in the intracellular space of β-cells. Cyanidin stimulated insulin secretion and increased intracellular Ca signals in a concentration-dependent manner. The Ca signals were abolished by nimodipine, an l-type voltage-dependent Ca channel (VDCC) blocker or under extracellular Ca free conditions. Stimulation of cells with cyanidin activated currents typical for VDCCs and up-regulated the expression of glucose transporter 2 (GLUT2), Kir, and Cav genes. Our findings indicate that cyanidin diffuses across the plasma membrane, leading to activation of l-type VDCCs. The increase in intracellular Ca stimulated insulin secretion and the expression of genes involved in this process. These findings suggest that cyanidin could be used as a promising agent to stimulate insulin secretion.

Publication Source (Journal or Book title)

Nutrients

Recommended Citation

Suantawee, T., Elazab, S. T., Hsu, W. H., Yao, S., Cheng, H., & Adisakwattana, S. (2017). Cyanidin Stimulates Insulin Secretion and Pancreatic β-Cell Gene Expression through Activation of l-type Voltage-Dependent Ca Channels. Nutrients, 9 (8) https://doi.org/10.3390/nu9080814