In Vitro Screening Method for Characterization of Macrophage Activation Responses

Authors

Brandon W. Lewis, Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Sonika Patial, Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Yogesh Saini, Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.

Document Type

Article

Publication Date

8-30-2022

Abstract

Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous or exogenous stimuli. Due to the existence of limitless stimuli and a multitude of receptors on macrophage surfaces, the nature of activation (or acquired functioning) can be specific to the encountering stimulus. This article describes a macrophage-activation screening platform in a 96-well format. The methodology involves the generation of bone marrow-derived macrophages, their activation into two extreme activation states, and screening of activated macrophages for expression of bonafide protein biomarkers. A high-throughput and stringent assay to determine macrophage activation markers developed in this article can be adapted for biomarker determination in pathological conditions and toxicant/drug safety screening.

Publication Source (Journal or Book title)

Methods and protocols

Recommended Citation

Lewis, B. W., Patial, S., & Saini, Y. (2022). In Vitro Screening Method for Characterization of Macrophage Activation Responses. Methods and protocols, 5 (5) https://doi.org/10.3390/mps5050068