Mouthwash as a non-invasive method of indocyanine green delivery for near-infrared fluorescence dental imaging

Authors

Zhongqiang Li, Louisiana State University, College of Engineering, Division of Electrical and Computer Engineering,, United States.
Zheng Li, Louisiana State University, College of Engineering, Division of Electrical and Computer Engineering,, United States.
Waleed Zaid, Louisiana State University Health Science Center, Oral and Maxillofacial Surgery, School of Dentistr, United States.
Michelle L. Osborn, Louisiana State University, School of Veterinary Medicine, Department of Comparative Biomedical Scie, United States.
Yanping Li, University of Saskatchewan, School of Environment and Sustainability, Saskatoon, Saskatchewan, Canada.
Shaomian Yao, Louisiana State University, School of Veterinary Medicine, Department of Comparative Biomedical Scie, United States.
Jian Xu, University of Saskatchewan, School of Environment and Sustainability, Saskatoon, Saskatchewan, Canada.

Document Type

Article

Publication Date

6-1-2022

Abstract

SIGNIFICANCE: X-ray imaging serves as the mainstream imaging in dentistry, but it involves risk of ionizing radiation. AIM: This study presents the feasibility of indocyanine green-assisted near-infrared fluorescence (ICG-NIRF) dental imaging with 785-nm NIR laser in the first (ICG-NIRF-I: 700 to 1000 nm) and second (ICG-NIRF-II: 1000 to 1700 nm) NIR wavelengths. APPROACH: Sprague Dawley rats with different postnatal days were used as animal models. ICG, as a fluorescence agent, was delivered to dental structures by subcutaneous injection (SC) and oral administration (OA). RESULTS: For SC method, erupted and unerupted molars could be observed from ICG-NIRF images at a short imaging time (<1  min). ICG-NIRF-II could achieve a better image contrast in unerupted molars at 24 h after ICG injection. The OA could serve as a non-invasive method for ICG delivery; it could also cause the glow-in-dark effect in unerupted molars. For erupted molars, OA can be considered as mouthwash and exhibits outstanding performance for delivery of ICG dye; erupted molar structures could be observed at a short imaging time (<1  min) and low ICG dose (0.05  mg  /  kg). CONCLUSIONS: Overall, ICG-NIRF with mouthwash could perform in-vivo dental imaging in two NIR wavelengths at a short time and low ICG dose.

Publication Source (Journal or Book title)

Journal of biomedical optics

Recommended Citation

Li, Z., Li, Z., Zaid, W., Osborn, M. L., Li, Y., Yao, S., & Xu, J. (2022). Mouthwash as a non-invasive method of indocyanine green delivery for near-infrared fluorescence dental imaging. Journal of biomedical optics, 27 (6) https://doi.org/10.1117/1.JBO.27.6.066001