An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage Exported Proteins Induces Sterilizing Protection against Infectious Sporozoite Challenge

Authors

Paul J. Rider, Division of Biotechnology and Molecular Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Mohd Kamil, Aly Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Ilknur Yilmaz, Aly Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Habibe N. Atmaca, Aly Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Merve Kalkan-Yazici, Microbiology Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Mehmet Ziya Doymaz, Microbiology Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Konstantin G. Kousoulas, Division of Biotechnology and Molecular Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Ahmed S. Aly, Aly Lab, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.

Document Type

Article

Publication Date

2-16-2022

Abstract

Here, we present the construction of an attenuated herpes simplex virus type-1 (HSV-1)-vectored vaccine, expressing three liver-stage (LS) malaria parasite exported proteins (EXP1, UIS3 and TMP21) as fusion proteins with the VP26 viral capsid protein. Intramuscular and subcutaneous immunizations of mice with a pooled vaccine, composed of the three attenuated virus strains expressing each LS antigen, induced sterile protection against the intravenous challenge of 17X-NL salivary gland sporozoites. Our data suggest that this malaria vaccine may be effective in preventing malaria parasite infection using practical routes of immunization in humans.

Publication Source (Journal or Book title)

Vaccines

Recommended Citation

Rider, P. J., Kamil, M., Yilmaz, I., Atmaca, H. N., Kalkan-Yazici, M., Ziya Doymaz, M., Kousoulas, K. G., & Aly, A. S. (2022). An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage Exported Proteins Induces Sterilizing Protection against Infectious Sporozoite Challenge. Vaccines, 10 (2) https://doi.org/10.3390/vaccines10020300