Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3 Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation

Authors

Weiqian Chen, Division of Rheumatology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Weishan Huang, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
Youqiu Xue, Division of Rheumatology, Department of Medicine, Pennsylvania State University Hershey College of Medicine, Hershey, PA, United States.
Ye Chen, Department of Clinical Immunology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Wenbin Qian, Division of Hematology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Jilin Ma, Division of Rheumatology, Department of Medicine, Pennsylvania State University Hershey College of Medicine, Hershey, PA, United States.
Avery August, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, LA, United States.
Julie Wang, Division of Rheumatology, Department of Medicine, Pennsylvania State University Hershey College of Medicine, Hershey, PA, United States.
Song Guo Zheng, Division of Rheumatology, Department of Medicine, Pennsylvania State University Hershey College of Medicine, Hershey, PA, United States.
Jin Lin, Division of Rheumatology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Document Type

Article

Publication Date

1-1-2022

Abstract

CD4Foxp3 regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced culture (iTreg) with different characteristics of suppressiveness, stability, and plasticity. There is an abundance of published data on neuropilin-1 (Nrp-1) as a tTreg marker, but little data exist on iTreg. The fidelity of Nrp-1 as a tTreg marker and its role in iTreg remains to be explored. This study found that Nrp-1 was expressed by a subset of Foxp3CD4T cells in the central and peripheral lymphoid organs in intact mice, as well as in iTreg. Nrp-1iTreg and Nrp-1iTreg were adoptively transferred into a T cell-mediated colitis model to determine their ability to suppress inflammation. Differences in gene expression between Nrp-1 and Nrp-1iTreg were analyzed by RNA sequencing. We demonstrated that the Nrp-1 subset of the iTreg exhibited enhanced suppressive function and stability compared to the Nrp-1 counterpart both and , partly depending on IL-10. We found that Nrp-1 is not an exclusive marker of tTreg, however, it is a biomarker identifying a new subset of iTreg with enhanced suppressive function, implicating a potential for Nrp-1iTreg cell therapy for autoimmune and inflammatory diseases.

Publication Source (Journal or Book title)

Frontiers in immunology

First Page

900139

Recommended Citation

Chen, W., Huang, W., Xue, Y., Chen, Y., Qian, W., Ma, J., August, A., Wang, J., Zheng, S. G., & Lin, J. (2022). Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3 Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation. Frontiers in immunology, 13, 900139. https://doi.org/10.3389/fimmu.2022.900139