Document Type
Patent
Patent Number
US 9206477B2
Abstract
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.
Application Number
US14/513,906
Assignees
Louisiana State University
Publication Date
12-8-2015
Recommended Citation
Barany, F., Barany, G., Hammer, R., Kempe, M., Blok, H., & Zirvi, M. (2015). Detection of Nucleic Acid Sequence Differences Using the Ligase Detection Reaction with Addressable Arrays. Retrieved from https://repository.lsu.edu/science_patents/38