Document Type

Article

Publication Date

1-1-1996

Abstract

Genetic uniformity was assessed among sweetpotato (Ipomoea batatas ) clones propagated through adventitious and nodal procedures. A single sprout each of 'Jewel.' 'Sumor,' and L87-95 was used as source of clonal plants that were simultaneously propagated through conventional adventitious procedures and a tissue culture-based nodal culture technique. A sample of 15 decamer primers generated 64 scorable amplified fragments in a PCR-based assay. 29 of which were putatively polymorphic across n = 60 samples ( 10 each of nodal and adventitiously derived plants/genotype). Within adventitiously derived materials, putative polymorphisms ranged from 4.7 <7r to>3U<£ depending on the genotypic class. In contrast, putative polymorphisms ranged from 0.0<£ to 3.1 **• among nodally derived samples. Marker loci differen-tiated genotypes as well as putative marker phenotype variants through a multidimensional scaling analysis of the genetic similarity matrix. An 'analysts of molecular variance' shows that genotypic effects accounted for 88.7°«• of the total molecular marker variability, w hue propagation effects ( within genotypic groups I accounted for 1U4T. Results confirm that clonal plants derived from preexisting meristematic regions arc more genetically uniform than plants propagated from adventitious origins.

Publication Source (Journal or Book title)

Journal of the American Society for Horticultural Science

First Page

170

Last Page

174

Download

Share

COinS