Microsatellite-based paternity analysis in polyploid sweetpotato
Document Type
Article
Publication Date
1-1-2002
Abstract
Using codominant molecular markers (microsatellites) for paternity identification was investigated in hexaploid sweetpotato [Ipomoea batatas (L.) Lam.]. Two experimental populations (CIP and LAES), each consisting of progeny of known parentage, were scored for the presence or absence of alleles segregating at IB-316 and IB-318 microsatellite loci. Paternity was assessed using paternity exclusion and the most-likely parent methods. In the former, paternity is assigned based on the identification of incompatible parent-progeny marker data. In contrast, the latter method incorporates paternity exclusion and a log-likelihood or LOD score that weighs progeny allelic patterns as to the likelihood that they could have come from a given paternal parent. The number of correctly allocated progeny differed for the methods. Paternity exclusion correctly allocated 7% and 25% of the progeny in the LAES and CIP populations, respectively. The most-likely parent method correctly allocated 23% and 88% of the progeny in the LAES and CIP populations, respectively. The greater misassignments in the LAES population were attributed to low allelic diversity at the LAES IB-318 locus and a larger parental population. This study demonstrates the feasibility of identifying paternity in sweetpotato using a minimal number of loci.
Publication Source (Journal or Book title)
Journal of the American Society for Horticultural Science
First Page
392
Last Page
396
Recommended Citation
Buteler, M., LaBonte, D., Jarret, R., & Macchiaveili, R. (2002). Microsatellite-based paternity analysis in polyploid sweetpotato. Journal of the American Society for Horticultural Science, 127 (3), 392-396. https://doi.org/10.21273/jashs.127.3.392
