An Alternative Method for Enzymatic Assay of Plant Invertases

Document Type

Article

Publication Date

1-1-1998

Abstract

An alternative coupled method was developed for the enzymatic assay of plant invertases. In this method, ADP produced from the phosphorylation of glucose and fructose (hydrolysis products of invertases) is coupled to oxidation of NADH by the enzymes pyruvate kinase and lactate dehydrogenase in the presence of phosphoenolpyruvate and NADH. This method was compared with the glucose-6-phosphate dehydrogenase method for both continuous and discontinuous assay by using purified invertase from baker's yeast and protein preparations derived from plant materials of three different species. This method is applicable to filtered and concentrated crude extracts. Statistical analysis indicated that the alternative method was similar in accuracy to the glucose-6-phosphate dehydrogenase method.

Publication Source (Journal or Book title)

Journal of Agricultural and Food Chemistry

First Page

3158

Last Page

3161

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