Variation in Isolate Virulence and Accession Resistance Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla in Soybean

Document Type

Article

Publication Date

1-1-2023

Abstract

Important stem and grain diseases of soybean (Glycine max [L.] Merr.) caused by Diaportheaspalathi, D. caulivora, and D. longicolla reduce yield in the United States. Sources of resistance to these pathogens have previously been reported; however, there is limited information regarding their resistance when exposed to geographically distinct isolates of the same species. In this study, four accessions from the USDA Soybean Germplasm Collection with reported resistance to D. aspalathi, D. caulivora, or D. longicolla were evaluated using geographically representative isolates within each species from the United States. For each fungus, a greenhouse experiment was conducted as a completely randomized design with a factorial arrangement (isolate rif accession). Plants were inoculated at the second to third of each Diaporthe species. Pathogenicity was assessed 21 days postinoculation as 0 = no lesion, 0.5 = lesion length?> 1 cm, and 1 = dead plant. A significant isolate-by-accession interaction (P < 0.05) was observed to affect pathogenicity as analyzed using nonparametric statistics (relative treatment effects [RTEs]), indicating that accessions responded differently to the isolates. Correlation analyses suggested that the RTEs on ‘Tracy-M’, ‘Dowling’, and ‘Crockett’ were weakly to moderately correlated with those of the D. aspalathi-susceptible ‘Bragg’, as well as for ‘PI567473B’ and ‘Century’ (D. caulivora), and ‘PI417507’ (D. longicolla), with the RTEs on ‘Hawkeye’ (P?> 0.05) indicating possible genetic variation for resistance within these accessions. Our results provide information related to the resistance of previously identified accessions to develop commercial cultivars with resistance to important pathogens within the genus Diaporthe.

Publication Source (Journal or Book title)

Plant Health Progress

First Page

482

Last Page

487

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