Document Type

Article

Publication Date

1-1-2007

Abstract

The reaction of sugarcane to Puccinia melanocephala Syd. & P. Syd. populations is intensely influenced by environmental conditions, make it difficult to conduct field evaluations of genotypes. This work aimed to develop a method for rust inoculation in leaf segments. The segments were obtained from 30-day-old plants of the susceptible variety SP70-1143. These were inoculated (2 × 104 viable spores/mL) and kept in test tubes. The treatments consisted of different incubation conditions, considering the variables (i) use or not of wet chamber after the inoculation; (ii) immersion of 1/4 or 3/4 of the section in the liquid contained in the test tube and; (iii) addition or not of benzimidazole in the water of the tube. The best treatment consisted of immersion of 3/4 of the section in test tubes containing water, with no moist chamber after the inoculation. After incubation (21 °C ± 1°C, 12 h of photoperiod) the sections remained viable for 20 days and showed good rust development. The method showed similar results when compared to the inoculation of whole plants of 3 varieties, making it possible to discriminate efficiently their reactions to rust.

Publication Source (Journal or Book title)

Fitopatologia Brasileira

First Page

253

Last Page

256

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