Development of a Particle Concentration Fluorescence Immunoassay for the Quantitative Determination of IgG in Bovine Milk
Document Type
Article
Publication Date
1-1-1993
Abstract
A rapid solid-phase particle concentration fluorescence immunoassay (PCFIA) was developed to quantitate the total IgG concentration in bovine milk. The assay is based on agglutination of antibody covalently bound to carboxyl-polystyrene particles with the antigen, which is in turn detected by a fluorescent labeled antibody by epifluorometry. Optimum assay performance was obtained using 0.25% w/v dilution of 0.8 µm carboxyl-polystyrene particles coated with 250 µg/mL of rabbit anti-bovine IgG at pH 4.5, two-step incubation of 30 and 20 min, respectively, and a 1:1000 dilution of the fluorescein isothiocyanate (FITC)-labeled rabbit anti-bovine IgG as the fluorescence signal. PCFIA is simple to perform, sensitive (5 ng of IgG/mL), accurate, repeatable, and faster than most immunoassays. © 1993, American Chemical Society. All rights reserved.
Publication Source (Journal or Book title)
Journal of Agricultural and Food Chemistry
First Page
682
Last Page
686
Recommended Citation
Losso, J., Kummer, A., Li-Chan, E., & Nakai, S. (1993). Development of a Particle Concentration Fluorescence Immunoassay for the Quantitative Determination of IgG in Bovine Milk. Journal of Agricultural and Food Chemistry, 41 (4), 682-686. https://doi.org/10.1021/jf00028a034