Thermally reversible hydrogel sheets for adult stem cell culture

Document Type

Conference Proceeding

Publication Date

12-1-2010

Abstract

A simple non-invasive method of cell retrieval using Methylcellulose (MC) coated Tissue Culture Poly-Styrone (TCPS) dishes was developed to allow the creation of single and multilayered adult stem cell sheet constructs while preserving the cell-cell and the cell-extracellular matrices. The optimal combination of MC-watersalt was found to be 12 to 14% of MC (mol. wt. of 15,000) in water with 0.5x PBS (∼150 mOsm). This solution exhibited a gel formation temperature of ∼32 C. The addition (evenly spread) of 200μl of 2mg/ml bovine collagen type -I (pH adjusted to 7.5) over the MC coated surface at 37 C, significantly improved the adult stem cell (ASC) adhesion and proliferation on the hydrogel system. Copyright © 2010 by ASME.

Publication Source (Journal or Book title)

ASME 2010 Summer Bioengineering Conference, SBC 2010

First Page

879

Last Page

880

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