Osteo-induction of human adipose derived stem cells cultured on poly (l-lactic acid) scaffolds prepared by thermally induced phase separation method

Document Type

Conference Proceeding

Publication Date

1-1-2015

Abstract

Bio-degradable Poly (l-lactic acid) (PLLA) scaffolds synthesized using thermally induced phase separation (TIPS) method was used to load cryo-preserved human adipose derived stem cells (hASCs). To make the scaffolds, PLLADioxane solutions were formed by dissolving PLLA in 1, 4-Dioxane with three different compositions (wt/vol). These PLLA-Dioxane solutions, frozen in three different cooling rates were lyophilized at 0.037bar and-70°C for 48hrs resulting in porous PLLA scaffolds. Based on the porosity, pore size and compressive strength, a suitable scaffold was chosen to investigate its bio-compatibility and osteo-inductive potential.

Publication Source (Journal or Book title)

ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)

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