Osteo-induction of human adipose derived stem cells cultured on poly (l-lactic acid) scaffolds prepared by thermally induced phase separation method
Document Type
Conference Proceeding
Publication Date
1-1-2015
Abstract
Bio-degradable Poly (l-lactic acid) (PLLA) scaffolds synthesized using thermally induced phase separation (TIPS) method was used to load cryo-preserved human adipose derived stem cells (hASCs). To make the scaffolds, PLLADioxane solutions were formed by dissolving PLLA in 1, 4-Dioxane with three different compositions (wt/vol). These PLLA-Dioxane solutions, frozen in three different cooling rates were lyophilized at 0.037bar and-70°C for 48hrs resulting in porous PLLA scaffolds. Based on the porosity, pore size and compressive strength, a suitable scaffold was chosen to investigate its bio-compatibility and osteo-inductive potential.
Publication Source (Journal or Book title)
ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Recommended Citation
Chinnasami, H., & Devireddy, R. (2015). Osteo-induction of human adipose derived stem cells cultured on poly (l-lactic acid) scaffolds prepared by thermally induced phase separation method. ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 3-2015 https://doi.org/10.1115/IMECE2015-51906