Adult Stem Cells Freezing Processes and Cryopreservation Protocols
Document Type
Article
Publication Date
1-1-2024
Abstract
The development of simple but effective storage protocols for adult stem cells will greatly enhance their use and utility in tissue-engineering applications. Cryopreservation has shown the most promise but is a fairly complex process, necessitating the use of chemicals called cryoprotective agents (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage.
Publication Source (Journal or Book title)
Methods in Molecular Biology
First Page
53
Last Page
89
Recommended Citation
Dey, M., & Devireddy, R. (2024). Adult Stem Cells Freezing Processes and Cryopreservation Protocols. Methods in Molecular Biology, 2783, 53-89. https://doi.org/10.1007/978-1-0716-3762-3_5
