Liquid-liquid segmented flows in polymer microfluidic channels for high throughput studies of enzyme activity using dual-color fluorescence crosscorrelation spectroscopy (FCCS)

Document Type

Conference Proceeding

Publication Date

1-1-2009

Abstract

We have designed and built a polymer-based microfluidic system for the realtime monitoring of enzyme activity by combining liquid-liquid two-phase flow with fluorescence cross-correlation spectroscopy (FCCS). Aqueous sample droplets were generated in a perfluorocarbon (FC 3283) carrier fluid with a nonionic fluoroussoluble surfactant (Perfluorooctanol, 10% v/v) in a polymer microchip operating in the capillary two-phase flow regime. The optical system was demonstrated to monitor controlled generation of highly regular droplets loaded with fluorescent beads at delivery rates ranging from 40 - 60 droplets s-1. Stable two-phase flow was promoted by rendering the walls of the microfluidic channel hydrophobic. © 2009 CBMS.

Publication Source (Journal or Book title)

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

First Page

952

Last Page

954

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