Degree

Doctor of Philosophy (PhD)

Department

Kinesiology

Document Type

Dissertation

Abstract

Hyperosmolar hyperglycemic syndrome (HHS) is a life-threating endocrine emergency commonly seen with type 2 diabetes. HHS has been identified to begin when hyperglycemia leads to glucosuria diuresis, which eventually leads to hyperosmotic conditions. However, hyperosmolality has not been considered as an initiator of HHS, although past research would suggest that it can initiate HHS, specifically through systemic inflammation and glucose insensitivity.

The first study examined the impact of exercise induced hypohydration effect on T-cell populations. After healthy participants reached hypohydration they were then rehydrated with water and monitored for 3-hours. Results indicated that rehydration attenuated the efflux of T-cells from the blood compartment, although at 3-hours post rehydration this attenuation was reversed as fluid was lost. This data indicates that fluid shifts influence T-cell mobility.

The second study examined hyperosmotic and HHS conditions effect on T-cells compared to normal osmotic conditions. Peripheral blood mononuclear cells (PBMCs) were cultured either simulated or non-stimulated in different osmotic and glycemic conditions. The results indicate that hyperosmotic conditions increase proinflammatory cytokine production, increases mitochondrial depolarization, and an increase in CD36/Glut-1 ratio indicating hyperosmotic conditions can potentially initiate HHS.

Date

11-2-2023

Committee Chair

Spielmann, Guillaume

Available for download on Sunday, November 01, 2026

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