Identifier

etd-07132006-173748

Degree

Doctor of Philosophy (PhD)

Department

Chemistry

Document Type

Dissertation

Abstract

Alzheimer's disease is a progressive neurological ailment affecting 4.5 million aging Americans. The disease is characterized by the presence in the brain of self-assembled fibrils consisting of beta amyloid protein (Aβ). Soluble Aβ protein is present in normal human cerebrospinal fluid, but it is unclear what makes the protein aggregate into insoluble plaques. There is evidence that the Aβ fibril assembly is affected by interactions with biological surfaces, such as neuronal membranes. Here, surfaces consisting of self-assembled alkanethiol monolayers with different end groups were used to test the effect of surface chemistry on the structure and morphology of aggregates formed from the Aβ peptide. Reflection-absorption infrared spectroscopy and scanning force microscopy (SFM) were used to examine the interactions of the protein with the monolayers. It was found that the surfaces have a seeding effect on the Aβ protein in solution and can actually induce aggregation of the Aβ protein over time. The outcomes are important, because the work described here is the first attempt at relating the chemical makeup of supported, model monolayer surfaces and their propensity to interact with Aβ peptide in solution. Patterned surfaces consisting of fouling and non-fouling monolayers were constructed to determine how these different areas would affect the deposition and aggregation of the Aβ protein. It appeared that the Aβ protein adsorbed onto the ethylene glycol even though the ethylene glycol-terminated monolayers alone were previously seen to be non-fouling. This was important because it appears that the fouling monolayer can induce a change in conformation that allows the protein to stick to what are normally non-fouling surfaces. Mica-supported lipid bilayers consisting of 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine (POPC) and 1-Oleoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-Glycero-3-Phosphocholine (NBD-PC) were also used as a simple cell membrane model system. Fluorescence photobleaching recovery and SFM were used to determine the effects of association of the fibrils with the bilayers. It was found that the Aβ peptide affected the fluidity of the lipid bilayers and inserted itself into the lipid bilayer. This experiment was important because it offers information about the insertion of the Aβ peptide into cell membranes that could potentially be toxic to cells.

Date

2006

Document Availability at the Time of Submission

Release the entire work immediately for access worldwide.

Committee Chair

Robin L. McCarley

DOI

10.31390/gradschool_dissertations.2681

Download

Included in

Chemistry Commons

Share

COinS