Identifier
etd-0801102-165734
Degree
Doctor of Philosophy (PhD)
Department
Chemistry
Document Type
Dissertation
Abstract
The visual determination of saccharides has been of interest for well over a century. Sugars are a relatively challenging class of compounds to analyze. They exhibit great structural similarity as well as transparency in the visible region. The sensing of specific saccharides could aid the monitoring of disease or industrial fermentation products. Herein I present new methods allowing for simple and rapid visual detection of sialic acid, fructose and a homologous series of prototypical neutral oligosaccharides, the linear maltodextrins, containing up to seven glucose residues. The new methods allow for selective color detection of fructose in a large excess of glucose and simultaneously determination of glucose. The fundamental problem of diminished optical detection of larger oligosaccharides is overcome using simple colorimetric agents. In addition, the first evidence that xanthenes form and serve as the active chromophores in resorcinarene solutions is presented. Meanwhile, the saccharide-induced optical signal transduction mechanism is investigated. A synthesis of resorcinarene derivatives, which have potential application in liposome stabilization, is also described.
Date
2002
Document Availability at the Time of Submission
Release the entire work immediately for access worldwide.
Recommended Citation
He, Ming, "Synthesis and study of new materials for the visual detection of biomolecules" (2002). LSU Doctoral Dissertations. 2124.
https://repository.lsu.edu/gradschool_dissertations/2124
Committee Chair
Robert M. Strongin
DOI
10.31390/gradschool_dissertations.2124