Diversity of gut bacteria of Reticulitermes flavipes as examined by 16S rRNA gene sequencing and amplified rDNA restriction analysis
Document Type
Article
Publication Date
9-1-2007
Abstract
The phylogenetic species richness of the bacteria in the gut of the termite Reticulitermes flavipes was examined using near full-length 16S rRNA gene sequencing and amplified rDNA restriction analysis (ARDRA). We amplified the genes by polymerase chain reaction (PCR) directly from a mixed population of termite gut bacteria and isolated them using cloning techniques. Sequence analysis of 42 clones identified a broad taxonomic range of ribotypes from six phyla within the domain Bacteria: Proteobacteria, Spirochaetes, Bacteroidetes, Firmicutes, Actinobacteria, and the recently proposed "Endomicrobia." Analysis of the sequence data suggested the presence of a termite specific bacterial lineage within Bacteroidetes. The ARDRA data included 261 different ARDRA profiles of 512 clones analyzed. These data suggest the gut flora in R. flavipes is extremely diverse. © 2007 Springer Science+Business Media, LLC.
Publication Source (Journal or Book title)
Current Microbiology
First Page
254
Last Page
259
Recommended Citation
Fisher, M., Miller, D., Brewster, C., Husseneder, C., & Dickerman, A. (2007). Diversity of gut bacteria of Reticulitermes flavipes as examined by 16S rRNA gene sequencing and amplified rDNA restriction analysis. Current Microbiology, 55 (3), 254-259. https://doi.org/10.1007/s00284-007-0136-8