Characterization of the plasma juvenile hormone esterase in synchronous last stadium female larvae of the sugar cane borer, Diatraea saccharalis (F.)

Document Type

Article

Publication Date

1-1-1983

Abstract

A peak in the plasma juvenile hormone esterase (JHE) activity occurred near the time when female last stadium sugar cane borers reached their maximum weight, and a second peak of plasma JHE activity and a peak in the plasma α-naphthyl acetate esterase (α-NAE) activity occurred at the prepupal stage. Two JHEs were resolved by high pressure liquid chromatography (HPLC), gel filtration, and isoelectric focusing. Their apparent mol. wts were 40,000 and 34,000 and their isoelectric points 6.9 and 6.3. Juvenile hormone I was metabolized by JHE at about twice the rate of JH III. The apparent Km of prepupal JHE and the Vmax were also greater for JH I (3.29 μM and 8.38 nM min-1 ml-1, respectively) than for JH III (3.04 μM and 3.75 nM min-1 ml-1, respectively). The last stadium JHE and α-NAE activity pattern, the inhibition profile, the enzyme thermal stability pattern, isoelectric focusing pattern, gel filtration profile, and HPLC analysis indicated that JH I and JH III were metabolized by the same JHEs, that were physiochemically distinct from general esterase activity (α-NAE activity). General esterase was relatively unimportant in the metabolism of unbound JH in the plasma of last stadium sugar cane borers. © 1983.

Publication Source (Journal or Book title)

Insect Biochemistry

First Page

163

Last Page

170

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