Title

Investigation of the reaction coordinate for ligand rebinding in photoexcited hemeproteins using transient Raman spectroscopy

Document Type

Article

Publication Date

1-1-1993

Abstract

We report pump-probe picosecond Raman experiments on oxy- and deoxyhemoglobin (oxyHb and deoxyHb, respectively) using 8 ps 532 nm pump pulses and 8 ps 355 nm probe pulses. Heme-to-protein vibrational cooling has been directly observed in deoxyHb for the first time, and the deconvolved cooling time constant is measured to be 2-5 ps. By applying our mode-specific Stokes and anti-Stokes technique to oxyHb, we find that any germinate recombination of photodeligated O2 must occur in either less than two picoseconds or longer than a nanosecond.

Publication Source (Journal or Book title)

Springer Series in Chemical Physics

First Page

528

Last Page

532

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