FACILE GENERATION OF 3D SPHEROIDS USING A THIOLACRYLATE HYDROGEL SCAFFOLD IN A MICROFLUIDIC DROPLET TRAPPING ARRAY
Document Type
Conference Proceeding
Publication Date
1-1-2021
Abstract
Three-dimensional (3D) cell culture better recapitulates in vivo conditions with 3D tumor spheroids serving as model systems to examine drug resistance in cancer. Droplet microfluidics offers a promising alternative to grow 3D spheroids from ~10 cells to reduce cellular heterogeneity which is lacking in other approaches that force >300 cells into a loose spheroid. This work utilizes a microfluidic droplet trapping array coupled with thiol-acrylate hydrogel as a scaffold to grow and interrogate 3D spheroids. As proof of concept, ER+ breast cancer spheroids are treated with fulvestrant followed by evaluation of proliferation and heterogeneity in the presence of estrogen.
Publication Source (Journal or Book title)
Microtas 2021 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
First Page
1031
Last Page
1032
Recommended Citation
Khan, A., Bajgiran, K., Moe, M., Lassiter, H., Dorman, J., Martin, E., Pojman, J., & Melvin, A. (2021). FACILE GENERATION OF 3D SPHEROIDS USING A THIOLACRYLATE HYDROGEL SCAFFOLD IN A MICROFLUIDIC DROPLET TRAPPING ARRAY. Microtas 2021 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1031-1032. Retrieved from https://repository.lsu.edu/chemistry_pubs/3986