Title

Magnetic Circular Dichroism Spectroscopy of Metalloproteins

Document Type

Article

Publication Date

1-1-2019

Abstract

Metals and metal clusters in proteins typically serve as important structural/functional motifs. Because of this reason, there is a wide range of techniques that specifically probe the structure and energy levels of metals in metalloproteins. One technique, magnetic circular dichroism (MCD) spectroscopy, is the focus of this chapter. MCD spectroscopy monitors the circular dichroism spectrum induced by a magnetic field and is an effective way of obtaining electronic and structural information of paramagnetic metal ions or clusters. The basic methodology of this technique is discussed along with examples of how MCD spectroscopy can be used to elucidate typical metal clusters in proteins. Special emphasis is placed on iron-sulfur (FeS) clusters.

Publication Source (Journal or Book title)

Methods in molecular biology (Clifton, N.J.)

First Page

213

Last Page

225

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