Title
Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla
Document Type
Article
Publication Date
1-1-2018
Abstract
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered "pseudo wild-type" enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.
Publication Source (Journal or Book title)
Methods in enzymology
First Page
33
Last Page
49
Recommended Citation
Gilbert, N. C., Neau, D. B., & Newcomer, M. E. (2018). Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla. Methods in enzymology, 605, 33-49. https://doi.org/10.1016/bs.mie.2018.02.010