Title
Design of Turn-On Near-Infrared Fluorescent Probes for Highly Sensitive and Selective Monitoring of Biopolymers
Document Type
Article
Publication Date
5-25-2020
Abstract
Simple, sensitive, and selective detection of specific biopolymers is critical in a broad range of biomedical and technological areas. We present a design of turn-on near-infrared (NIR) fluorescent probes with intrinsically high signal-to-background ratio. The fluorescent signal generation mechanism is based on the aggregation/de-aggregation of phthalocyanine chromophores controlled by selective binding of small-molecule "anchor" groups to a specific binding site of a target biopolymer. As a proof-of-concept, we demonstrate a design of a sensor for EGFR tyrosine kinase-an important target in cancer research. The universality of the fluorescent signal generation mechanism, as well as the dependence of the response selectivity on the choice of the small-molecule "anchor" group, make it possible to use this approach to design reliable turn-on NIR fluorescent sensors for detecting specific protein targets present in the low-nanomolar concentration range.
Publication Source (Journal or Book title)
Angewandte Chemie (International ed. in English)
First Page
8440
Last Page
8444
Recommended Citation
Ducharme, G. T., LaCasse, Z., Sheth, T., Nesterova, I. V., & Nesterov, E. E. (2020). Design of Turn-On Near-Infrared Fluorescent Probes for Highly Sensitive and Selective Monitoring of Biopolymers. Angewandte Chemie (International ed. in English), 59 (22), 8440-8444. https://doi.org/10.1002/anie.202000108
