Title

A dual input DNA-based molecular switch

Document Type

Article

Publication Date

11-1-2014

Abstract

We have designed and characterized a DNA-based molecular switch which processes two physiologically relevant inputs: pH (i.e. alkalinisation) and enzymatic activity, and generates a chemical output (in situ synthesized oligonucleotide). The design, based on allosteric interactions between i-motif and hairpin stem within the DNA molecule, addresses such critical physiological system parameters as molecular simplicity, tunability, orthogonality of the two input sensing domains, and compatibility with intracellular operation/delivery.

Publication Source (Journal or Book title)

Molecular bioSystems

First Page

2810

Last Page

4

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