Title
Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation
Document Type
Article
Publication Date
4-1-2002
Abstract
Carbon nanotubes are functionalized by bovine serum albumin (BSA) proteins via diimide-activated amidation under ambient conditions. The nanotube-BSA conjugates thus obtained are highly water-soluble, forming dark-colored aqueous solutions. Results from characterizations using atomic force microscopy (AFM), thermal gravimetric analysis, Raman, and gel electrophoresis show that the conjugate samples indeed contain both carbon nanotubes and BSA proteins and that the protein species are intimately associated with the nanotubes. Bioactivities of the nanotube-bound proteins are evaluated using the total protein micro-determination assay (the modified Lowry procedure). The results show that the overwhelming majority (∼90%) of the protein species in the nanotube-BSA conjugates remain bioactive.
Publication Source (Journal or Book title)
Nano Letters
First Page
311
Last Page
314
Recommended Citation
Huang, W., Taylor, S., Fu, K., Lin, Y., Zhang, D., Hanks, T., Rao, A., & Sun, Y. (2002). Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation. Nano Letters, 2 (4), 311-314. https://doi.org/10.1021/nl010095i