Title

Fluorescence-detected circular dichroism studies of serum albumins

Document Type

Article

Publication Date

8-1-1987

Abstract

Multidimensional fluorescence-detected circular dichroism (FDCD) is used to investigate the binding of bilirubin to four mammalian serum albumins. It is shown that the complexes formed with the albumins have distinctly different FDCD spectra. The effect of the pH-dependent transition from basic to acidic conditions on the complexes is examined. The binding of warfarin to albumin is also presented to demonstrate the general analytical utility of the multidimensional FDCD measurement for biochemical analysis. © 1987.

Publication Source (Journal or Book title)

Analytical Biochemistry

First Page

466

Last Page

473

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