Document Type
Article
Publication Date
3-1-2006
Abstract
We report on the fabrication and performance of a gel microfluidic chip interfaced to laser desorption/ionization (LDI) mass spectrometry with a time-of-flight mass analyzer. The chip was fabricated from poly(methylmethacrylate) with a poly(dimethyl siloxane) cover. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed in the channel of the microfluidic chip. After electrophoresis, the cover was removed and either the PDMS chip or the PMMA cover was mounted in a modified MALDI ion source for analysis. Ions were formed by irradiating the channel with 2.95 μm radiation from a pulsed optical parametric oscillator (OPO), which is coincident with IR absorption by N-H and O-H stretch of the gel components. No matrix was added. The microfluidic chip design allowed a decrease in the volume of material required for analysis over conventional gel slabs, thus enabling improvement in the detection limit to a pmol level, a three orders of magnitude improvement over previous studies in which desorption was achieved from an excised section of a conventional gel. © 2006 American Society for Mass Spectrometry.
Publication Source (Journal or Book title)
Journal of the American Society for Mass Spectrometry
First Page
469
Last Page
474
Recommended Citation
Xu, Y., Little, M., & Murray, K. (2006). Interfacing capillary gel microfluidic chips with infrared laser desorption mass spectrometry. Journal of the American Society for Mass Spectrometry, 17 (3), 469-474. https://doi.org/10.1016/j.jasms.2005.12.003
