"Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite " by Cornelia Rosu, Rafael Cueto et al.

Faculty Publications

Title

Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite Particles through the Photoinduced Cross-Linking of Unmodified Proteins Method

Authors

Cornelia Rosu, School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
Rafael Cueto, Department of Chemistry and Macromolecular Studies Group, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Paul S. Russo, School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

Document Type

Article

Publication Date

8-23-2016

Abstract

Photoinduced cross-linking of unmodified proteins, PICUP, was extended to core-shell silica-polypeptide composite particles to produce poly(colloid)s. Silica particles coated with poly(l-tyrosine), PTYR-SiO2, served as the monomer units. The PICUP reaction accomplished the formation of dityrosil linkages between the tyrosine units by illumination of photo-oxidizing ruthenium(II) bipyridyl catalyst under physiological conditions. The PICUP method was compared with an enzymatic route intermediated by horseradish peroxidase as catalyst. The PTYR-SiO2 particles feature high PTYR content in the shell, which facilitated the formation of heavily cross-linked but unstructured aggregates. After magnetic alignment of superparamagnetic PTYR-SiO2-cobalt composite particles, only the PICUP approach enabled the preparation of isolated chain-like poly(colloid)s. The cross-linking products were confirmed by FTIR. The native secondary structure of poly(l-tyrosine) is preserved in these poly(colloid)s. Because the PICUP reaction does not require the modification of the polypeptide structure, the cross-linked PTYR will retain its characteristic functions as a poly(amino acid). The PICUP method opens the door to a variety of PTYR-based poly(colloid) architectures.

Publication Source (Journal or Book title)

Langmuir : the ACS journal of surfaces and colloids

First Page

8392

Last Page

402

Recommended Citation

Rosu, C., Cueto, R., & Russo, P. S. (2016). Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite Particles through the Photoinduced Cross-Linking of Unmodified Proteins Method. Langmuir : the ACS journal of surfaces and colloids, 32 (33), 8392-402. https://doi.org/10.1021/acs.langmuir.6b01815

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