Title
Fluorometric Characterization of DUB Activity: From Single-Enzyme Reactions to Live Intact Cells
Document Type
Article
Publication Date
1-1-2023
Abstract
Fluorescently tagged molecular probes capable of time- and concentration-dependent quantification of deubiquitinating enzyme (DUB) activity allow for precise characterization of both enzyme and DUB inhibitor. These probes are compatible with most plate readers allowing for rapid, facile fluorometric analysis of DUB activity. DUB activity can be measured in purified enzyme reactions, in cell lysates, or in intact cells depending upon the choice of the fluorometric probe. This chapter describes protocols and potential analysis tools to investigate DUB activity in these three scenarios.
Publication Source (Journal or Book title)
Methods in molecular biology (Clifton, N.J.)
First Page
25
Last Page
44
Recommended Citation
Rahnama, A., & Melvin, A. T. (2023). Fluorometric Characterization of DUB Activity: From Single-Enzyme Reactions to Live Intact Cells. Methods in molecular biology (Clifton, N.J.), 2591, 25-44. https://doi.org/10.1007/978-1-0716-2803-4_3