"Northern Blot Detection of Virus-Derived Small Interfering RNAs in Cae" by Tianyun Long and Rui Lu

Faculty Publications

Title

Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

Authors

Tianyun Long, Department of Biological Sciences, Louisiana State University, 202 Life Science Building, Baton Rouge, LA, 70803, USA.
Rui Lu, Department of Biological Sciences, Louisiana State University, 202 Life Science Building, Baton Rouge, LA, 70803, USA. ruilu@lsu.edu.

Document Type

Article

Publication Date

1-1-2017

Abstract

Northern blot analysis has been widely used as a tool for detection and characterization of specific RNA molecules. When coupled with radioactive probe northern blot allows for robust detection and characterization of small RNA molecules of trace amount. Here, we describe the detection and size characterization of virus-derived small interfering RNAs (vsiRNAs) in C. elegans using nonradioactive DNA oligo probes in northern blotting. Our protocol allows for the detection and characterization of not only primary vsiRNAs but also secondary vsiRNAs, a class of single-stranded vsiRNAs that has distinct migration pattern, and can be easily adapted to the detection of vsiRNAs in other organisms.

Publication Source (Journal or Book title)

Methods in molecular biology (Clifton, N.J.)

First Page

Last Page

Recommended Citation

Long, T., & Lu, R. (2017). Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes. Methods in molecular biology (Clifton, N.J.), 1656, 79-88. https://doi.org/10.1007/978-1-4939-7237-1_3

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