"Intracellular localization of group 3 LEA proteins in embryos of Artem" by Leaf C. Boswell and Steven C. Hand

Faculty Publications

Title

Intracellular localization of group 3 LEA proteins in embryos of Artemia franciscana

Authors

Leaf C. Boswell, Division of Cellular, Developmental and Integrative Biology, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, United States. Electronic address: lboswe1@lsu.edu.
Steven C. Hand, Division of Cellular, Developmental and Integrative Biology, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, United States.

Document Type

Article

Publication Date

12-1-2014

Abstract

Late embryogenesis abundant (LEA) proteins are accumulated by anhydrobiotic organisms in response to desiccation and improve survivorship during water stress. In this study we provide the first direct evidence for the subcellular localizations of AfrLEA2 and AfrLEA3m (and its subforms) in anhydrobiotic embryos of Artemia franciscana. Immunohistochemistry shows AfrLEA2 to reside in the cytoplasm and nucleus, and the four AfrLEA3m proteins to be localized to the mitochondrion. Cellular locations are supported by Western blots of mitochondrial, nuclear and cytoplasmic fractions. The presence of LEA proteins in multiple subcellular compartments of A. franciscana embryos suggests the need to protect biological structures in many areas of a cell in order for an organism to survive desiccation stress, and may explain in part why a multitude of different LEA proteins are expressed by a single organism.

Publication Source (Journal or Book title)

Tissue & cell

First Page

514

Last Page

Recommended Citation

Boswell, L. C., & Hand, S. C. (2014). Intracellular localization of group 3 LEA proteins in embryos of Artemia franciscana. Tissue & cell, 46 (6), 514-9. https://doi.org/10.1016/j.tice.2014.09.004

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