Title
Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease
Document Type
Article
Publication Date
2-1-2002
Abstract
An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.
Publication Source (Journal or Book title)
Journal of bacteriology
First Page
1003
Last Page
9
Recommended Citation
Earl, A. M., Rankin, S. K., Kim, K., Lamendola, O. N., & Battista, J. R. (2002). Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease. Journal of bacteriology, 184 (4), 1003-9. https://doi.org/10.1128/jb.184.4.1003-1009.2002