Document Type

Article

Publication Date

8-1-2009

Abstract

The Arabidopsis thaliana mutant psbo1 has recently been described and characterized. Loss of expression of the PsbO-1 protein leads to a variety of functional perturbations including elevated levels of the PsbO-2 protein and defects on both the oxidizing- and reducing-sides of Photosystem II. In this communication, two plant lines were produced using the psbo1 mutant as transgenic host, which contained an N-terminally histidine6-tagged PsbO-1 protein. This protein was expressed and correctly targeted into the thylakoid lumen. Immunological analysis indicated that different levels of expression of the modified PsbO-1 protein were obtained in different transgenic plant lines and that the level of expression in each line was stable over several generations. Examination of the Photosystem II closure kinetics demonstrated that the defective double reduction of QB and the delayed exchange of QBH2 with the plastoquinone pool which were observed during the characterization of the psbo1 mutant were effectively restored to wild-type levels by the His6-tagged PsbO-1 protein. Flash fluorescence induction and decay were also examined. Our results indicated that high expression of the modified PsbO-1 was required to increase the ratio of PS IIα/PS IIβ reaction centers to wild-type levels. Fluorescence decay kinetics in the absence of DCMU indicated that the expression of the His6-tagged PsbO-1 protein restored efficient electron transfer to QB, while in the presence of DCMU, charge recombination between QA- and the S2 state of the oxygen-evolving complex occurred at near wild-type rates. Our results indicate that high expression of the His6-tagged PsbO-1 protein efficiently complements nearly all of the photochemical defects observed in the psbo1 mutant. Additionally, this study establishes a platform on which the in vivo consequences of site-directed mutagenesis of the PsbO-1 protein can be examined. © 2009 Elsevier B.V. All rights reserved.

Publication Source (Journal or Book title)

Biochimica et Biophysica Acta - Bioenergetics

First Page

1029

Last Page

1038

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