An integrated metric physical map of human chromosome 19

Linda K. Ashworth, Lawrence Livermore National Laboratory
Mark A. Batzer, Lawrence Livermore National Laboratory
Brigitte Brandriff, Lawrence Livermore National Laboratory
Elbert Branscomb, Lawrence Livermore National Laboratory
Pieter de Jong, Lawrence Livermore National Laboratory
Emilio Garcia, Lawrence Livermore National Laboratory
Jeffrey A. Garnes, Lawrence Livermore National Laboratory
Laurie A. Gordon, Lawrence Livermore National Laboratory
Jane E. Lamerdin, Lawrence Livermore National Laboratory
Greg Lennon, Lawrence Livermore National Laboratory
Harvey Mohrenweiser, Lawrence Livermore National Laboratory
Anne S. Olsen, Lawrence Livermore National Laboratory
Tom Slezak, Lawrence Livermore National Laboratory
Anthony V. Carrano, Lawrence Livermore National Laboratory

Abstract

A metric physical map of human chromosome 19 has been generated. The foundation of the map is sets of overlapping cosmids (contigs) generated by automated fingerprinting spanning over 95% of the euchromatin, about 50 megabases (Mb). Distances between selected cosmid clones were estimated using fluorescence in situ hybridization in sperm pronuclei, providing both order and distance between contigs. An average inter–marker separation of 230 kb has been obtained across the non–centromeric portion of the chromosome. Various types of larger insert clones were used to span gaps between contigs. Currently, the map consists of 51 ‘islands’ containing multiple clone types, whose size, order and relative distance are known. Over 450 genes, genetic markers, sequence tagged sites (STSs), anonymous cDNAs, and other markers have been localized. In addition, EcoRI restriction maps have been generated for >41 Mb (-83%) of the chromosome. © 1995 Nature Publishing Group.