Isolation and characterization of two new methane-producing cocci: Methanogenium olentangi, sp. nov., and Methanococcus deltae, sp. nov.
Abstract
Two new methane-producing cocci have been isolated from separate sediment enrichments using a defined synthetic growth medium. A freshwater methanogen, Methanogenium olentangyi, sp. nov., was isolated from Olentangy River sediment, Columbus, Ohio. The organism was a non-motile, irregular coccus that produced methane from CO2 plus H2, and required acetate for growth. A marine methanogen, Methanococcus deltae, sp. nov., was isolated from Gulf of Mexico sediments and was a non-motile, irregular coccus that grew on CO2 plus H2, or on formate. The guanine-plus-cytosine content of the deoxyribonucleic acid of the isolates was 54.4 and 40.5 mol%, respectively. © 1983 Springer-Verlag.